055 HYPOXIA CONDITIONS DIFFERENTIALLY MODULATE NORMAL AND OSTEOARTHRITIC HUMAN ARTICULAR CHONDROCYTE PROTEOMES

Evaluation of CD98 Expression in Normal and Osteoarthritic Human Articular Chondrocytes

Background: Recent studies have provided evidence that integrins play roles in recognition of mechanical stimuli and its translation into a cellular response. Integrin signaling may be regulated by a number of mechanisms including accessory proteins such as CD98 (4F2 antigen). Objectives: To determine CD98 expression by human articular chondrocytes and its involvement in human articular mechano...

Differential Immunohistochemical Expression Pattern of Galectin-3 in Normal and Osteoarthritic Human Articular Cartilage

Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones.   Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied.   Methods: Using immunohistochemistry and standard we...

Chondrocyte multiplication in osteoarthritic articular cartilage.

The Expression of Signal Regulatory Protein-alpha in Normal and Osteoarthritic Human Articular Cartilage and Its Involvement in Chondrocyte Mechano-transduction Response

Signal regulatory proteins (SIRP) belong to immunoglobulin super family (IgSF) and relate to integrin signaling cascades. It has been shown that SIRPa is expressed in a variety of cells including myeloid cells and neurons. In the present study the expression of this IgSF member in articular chondrocytes was investigated. Methods: Using a panel of anti-SIRPalpha antibodies, immunohistochemistry...

Chondrocyte number and proteoglycan synthesis in the aging and osteoarthritic human articular cartilage.

OBJECTIVE To correlate the number of chondrocytes in healthy and osteoarthritic human articular cartilage with age, and to evaluate the influence of donor age on total proteoglycan synthesis. METHODS Chondrocytes were isolated from human articular cartilage derived from hip joints with and without osteoarthritic lesions. The cell number was normalised to cartilage sample wet weight. In additi...